ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (1): 190-197.doi: 10.11843/j.issn.0366-6964.2016.01.026

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1H-NMR-based Plasma Metabolic Profiling of Postpartum Dairy Cows with Ovarian Inactivity

XU Chu-chu,XIA Cheng*,SUN Yu-hang,XIAO Xin-huan,WANG Gang,SHU Shi,ZHANG Hong-you,XU Chuang,YANG Wei   

  1. (College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
  • Received:2015-06-10 Online:2016-01-23 Published:2016-01-23

Abstract:

To understand the differences in metabolic changes between cows with ovarian inactivity and estrus cows,we selected cows at 60-90 d postpartum,which had similar age,parity,body condition score and milk yield.According to clinical manifestations,B-ultrasound scan,rectal examination and hormone tests,10 cows were assigned to the estrus group (A) and 10 to the ovarian inactivity group (B).All plasma samples were analyzed by 1H NMR spectroscopy to compare plasma metabolomic profiles between the groups.We used multivariate pattern recognition,to screen for different metabolites in plasma of anestrus cows.Compared with normal estrous cows,there were abnormalities in 12 kinds of metabolites in postpartum cows with ovarian inactivity,including an increase in acetic acid,citric acid and tyrosine,and a decrease in low-density lipoprotein,very low density lipoprotein,lipids,alanine,pyruvate,creatine,choline,phosphorylcholine and glycerophosphorylcholine.These metabolites were closely related to abnormality of glucose,amino acid,lipoprotein and choline metabolism,which may disturb the normal estrus.The decrease in plasma creatine and the increase in tyrosine were new changes for ovarian inactivity of postpartum cows.In conclusion,a variety of metabolic disorders indicate that ovarian inactivity is related to negative energy balance in early lactation,and has the risk of ketosis and fatty liver.The decrease of creatine and choline concentration and increase of p-hydroxyphenylalanine concentration in cows with ovarian inactivity provide a new direction for research on the mechanism of ovarian inactivity in cows.

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